Skip to content

Composing the Volume ​

Composing turns the placements into a single output volume. It lives in the Align & Compose step: review the placement summary in the toolbar, set the output options, and press Compose; the stitch is streamed slice by slice and the recipe is saved alongside the result.

Placement Summary ​

  • The placement summary lists every stack with its z offset, dx, dy, rotation, and the slice range kept

  • A per-junction note shows the inferred relationship: "continues in z", "continues in z, merged", or "side by side"

  • The mode line notes how seams are handled: images get feathered seams; label maps use nearest-neighbor with hard seams by design

Options ​

  • Match intensities between stacks: rescales each stack to the reference stack's mean and standard deviation; recommended when stacks came from separate sessions. Grayscale only, so this option is hidden for label maps

  • Crop to common area: outputs only the xy region all stacks share, instead of a union canvas with the gaps filled

  • Output name: the basename of the stitched TIFF

Output and Recipe ​

  • The output is a multi-page TIFF written to the results area; its size and dtype are reported on completion

  • A recipe (stitch_recipe.json) is saved next to the output with workspace-relative paths, ready to reapply to sibling volumes

  • If any duplicated sections were merged, the result reports how many were averaged

  • Open the result directly in the Image Viewer, or start a new run

Warnings ​

The composer reports issues rather than failing silently:

  • dtype mismatch between a stack and the reference (it is cast to the reference dtype)

  • In label maps mode, a merge flag ignored because class IDs cannot be averaged

  • Label sets that differ from the reference (classes present in a stack but not the reference, sampled at the junction slices)

  • z gaps: output slices with no contributing stack, which are filled

  • Overlapping kept ranges that were averaged even though no stack was flagged to merge, which usually means a hand-edited recipe

Part of DFG Priority Programme SPP2332 "Physics of Parasitism"